2D Differentiation and Induction Protocols
Motor Neuron Induction and Differentiation
This protocol is an interactive and clickable pdf describing how to:
• Generate motor neural progenitor cells (MNPCs)
• Generate motor neurons from MNPCs
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Dopaminergic or Cortical Neuron Differentiation
This is an interactive protocol to describe and to assist in training on how to:
• Culture dopaminergic (DA) or cortical neural progenitor cells (NPCs)
• Generate DA or cortical neurons from DA or cortical NPCs, respectively
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Induction of Dopaminergic Neuronal Progenitors From iPSCs Using Microfabricated Disk
This protocol describes how to generate dopaminergic neural progenitor cells (DPCs) from induced-pluripotent stem cells (iPSCs) using microfabricated embryoid body disk device (EB disk).
This protocol describes how to:
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Thaw and culture dopaminergic neural progenitor cells (DPCs).
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Plate DPCs in a 96 well format to be differentiated into dopaminergic neurons (DNs)
Cilia, iPSC-derived Neurons, and Neurodevelopmental Disorders
Watch this video to learn about the work being done at the EDDU with iPSC-derived neurons and brain organoids to understand the role of cilia in neurodevelopmental disorders.